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This paper describes the results obtained when the characteristics of metabolic process changes in leukemia, including brucellosis-complicated leukemia, were studied. To do it, 50 blood serum samples were taken from cows with specific antibodies against bovine leukemia virus (BLV) under the results of immunological diffusion reactions (IDR), indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA). All samples were serologically tested to detect a number of bacterial infections resulted in a possibility to establish that the BLV carrier state in most animals is combined with a bacterial infection, especially brucellosis (46%), chlamydia (20%), paratuberculosis (12%) and campylobacteriosis (8 %). At the next stage, 3 groups of 10 animals each were formed to study the metabolic process level, i.e. clinical healthy animals with no specific antibodies revealed during diagnostic tests for leukemia and other infections (Group 1); BLV carriers (Group 2); brucellosisand virus carrier animals (Group 3). Analysis of the blood chemistry values obtained for the experimental groups showed an uneven path of changes, especially for the protein and fat metabolism parameters. The difference was in albumin and cholesterol concentrations reduced in BLV infected animals, while their level was significantly increased in animals with leukemia associated with brucellosis, on the contrary.

The development of animal husbandry suffers various kinds of losses due to the spread of infectious diseases among animals, in particular Brucellosis. A challenge faced by Brucella researchers has been the accurate identification of new isolates within the genus while preserving sufficient, and not excessive, biosafety and biosecurity requirements. The availability of discriminatory molecular typing tools to inform and assist conventional epidemiological approaches would be invaluable in controlling these infections, but efforts have been hampered by the genetic homogeneity of the genus. In this work, for better identification of infection, for control and monitor the source of outbreaks in prosperous areas was carried out identification of Brucella spp. strains which circulating in the Kostanay region. For this was used using multilocus analysis of a variable number of tandem repeats sequenced by 16 s – PNK on a genetic analyzer (sequencer). According to the results of a study of cattle, cultures of microorganisms were infected: No. 4, 5, 7, 8. Comparison of the obtained results with similar results of domestic and foreign works by A. Shevtsov, G. Borrello, P. Le Fleche, G. Garofolo suggest that the genotyping of local strains has an importance in the molecular epizootology of the Republic of Kazakhstan.