Religion and its practice have been found to significantly influence marital satisfaction and quality. However, there is a paucity of research that has explored these relationships among married adults living in the Caribbean and Latin America. This study examines the influence of prayer and family worship on relationship satisfaction, emotional attunement, conflict resolution and belief in traditional gender roles among Christian Caribbean and Latin American married adults. Data were gathered from 3997 Seventh-day Adventist (SDA) adults in the Caribbean and Latin America. Hierarchical multiple regression (HMR) analyses were conducted to evaluate the associations between religious practices (personal prayer, family worship, church attendance, and bible study) and relationship outcomes (relationship satisfaction, emotional attunement, conflict resolution, beliefs in traditional roles) independent of gender and level of education. The results suggest that married adults who spend more time in habitual prayer and at least weekly family worship experience higher levels of relationship satisfaction, emotional attunement and satisfaction with how conflicts were resolved. Additionally, married adults who were more likely to have a daily prayer life also reported having more egalitarian beliefs about roles and responsibilities in their relationships. These findings highlight the significant influence prayer and family worship have on relationship satisfaction and functioning.