A total of 20 agricultural organic materials including inorganic compounds, plant oils and plant extracts were used in the study for assessing the control efficacy on pepper diseases. Among inorganic compounds, only copper hydroxide showed inhibitory effect on both Phytophthora capsici causing Phytophthora blight and Colletotrichum acutatum causing anthracnose. Phosphorous acid inhibited the growth of P. capsici on PDA, and Sulfur/quicklime had it on that of C. acutatum. Plant essential oil, rosemary oil, and rapeseed oil among plant oils and plant extract of Japanese apricot/ginkgo nut inhibited the mycelial growth of the two pathogens. In the screening using pepper plant seedlings, the control efficacy on Phytophthora blight in 6-leaf stage of seedling was superior to that in 4-leaf stage of seedling. A protective effect on Phytophthora blight was displayed by copper hydroxide, sulfur/quicklime, water soluble calcium, phosphorous acid, plant essential oil, and cloves extract. When C. acutatum was inoculated by the non-wound method, copper hydroxide and rapeseed oil showed excellent protective activities with control values of 91.3% and 82.6%, respectively. However, copper hydroxide did not show any activity, when C. acutatum was inoculated after wounding pepper fruits. All organic materials never showed the curative effect on Phytophthora blight and anthracnose in pepper seedling assay and fruit assay.
It was conducted to evaluate the control efficacy of Bordeaux mixture on pepper anthracnose in vitro and in vivo. The suppressive efficacy of Bordeaux mixture on mycelial growth and spore germination of Colletotrichum acutatum was investigated on PDA, cellophane membranes and pepper fruits, respectively. Furthermore, its control value was evaluated on detached pepper fruits inoculated with C. acutatum by wound and non-wound inoculation method, and in fields. The mycelial growth of C. acutatum JC24 was inhibited by 90.0% on PDA amended with 937 µg mL −1 of Bordeaux mixture. While the spore germination of C. acutatum JC24 was inhibited perfectly on cellophane membranes treated at 187 µg mL −1 of Bordeaux mixture, that on fruits inoculated with the pathogen by wound inoculation and non-wound inoculation method was inhibited by 88.1% and 91.3%, respectively. Although the control value on fruits treated with 937 µg mL −1 of Bordeaux mixture was 17.6% in wound inoculation method, it was 58.8% in non-wound inoculation method. In fields, when Bordeaux mixture was sprayed five times at 14 day-intervals, it showed 55.7% and 61.7% of control value in 2012 and 2013, respectively. We think Bordeaux mixture was able to use as an eco-friendly organic farming material to control pepper anthracnose based on the above-mentioned results.
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