Salmo trutta caspius population is an important species for aquaculture and livestock industry. Moreover, these species are used for studies on molecular markers. Mitochondrial genomic is also beneficial for phylogenetic studies in salmonid species. They are applied for maternal traits whereas paternal traits are related to nuclear genomics. Mtgenomics will be genetically highly divergent indicating that they may represent distinct and potentially locally adapted gene pools. Evolutionary history of the salmo taxa such as brown trout, salmo salar, and s. trutta populations has been studied. In the present study, 50 samples of the Iranian S.t. caspius population were collected from three regions [Tonekabon (Cheshmekileh Roud), Ramsar (Safa Roud), and Talesh (Nav roud)]. The PCR product was carried out for machinery sequencing. Mtgenomic in S.t. caspius was deposited in GenBank under accession no. LC011387.1. Evolutionary analyses were conducted using MEGA7 software. The analysis involved 50 nucleotide sequences. A close relationship was observed between the samples.