The protccliw: effect of glutatltioue (GSH) a11d granulocyte colony stimulating factor (G-CSF) 011 myelosuppression inducet! by 5-fluorouraci/ (5-FU) were compared in female mice. Tfte animals were divided into seven gro11ps. Group I (10 mice) received no treotment. group 2 (/0 mice) received GSH (800 mgl kg) by inrmperi10neal rotae in daily doses for rlre first 7 days thell left untreated for cmotfler 7 days, a11d ~JI'OIIJI J (10 mice) received G-CSF (250 11glkg) by subcuta11e0tts route in daily doses for the first 7 days tlt('/1 lc:ft tmtreated for u1eotl!a 7 days. Animnls iu sroups I. 2 wu! J were sacrificed on day 15. Group 4 (20 mice} received a single dose-of 5-FU (160 mg!kg) by ;,ztraperiloneal route in t!le stft day, Group 5 (20 mict:) reccil'ctl GS/1 i11 dnily doses jrJr the first 7 doy.v followed by n .ingle dose of 5-FU in the 8 111 dny, Graul' 6 (20 mict:) received G-CSF i11 daily doses fortlte first 7 days followed by o single dose of 5-FU in the 8'11 dny and group 7 o{animnls (20 mice) rei:eit>ed da ily doses of botft GSH t1nd G-CSF for tl1e first 7 days follo wed by a single Hose of 5-FU in the 8tll dny. Animals in groups 4, 5. 6 and 7 were di1•icled into 2 .wbgroups; subgroup a: 10 m ice were sacrificed 011 day 9 i. e. I clay after 5-FU adminislrutiou nnd subgroup b: 10 mice were sacrificed 0 11 day 15 i.e. !week after 5-FU administratio11. Mice were sacrificed by Clll throat all({ blood sainples were obtni11ed for determination of haemoto!ogical values; lwemoglvbilt (Hb). luwmatocrite value (liCT). mean corpuscular haemoglobin concentration (MCHC). mean corpit.~culur l'olume (MCV), 'mean corpuscularlwemoglobin (MCH), as well as total (TLC) and tlif fen:ntinl (DLC) leucocyte cowtt. Theu dissection of mice was done •where the right femurs were used for bon<' '"''rmll' cytology, w(,ile left femurs were used for bone marrow histopathology. In groups 5o given GSII pretreutmellt aud 6a gi ven G •CSF pretreatme11t (where mice killed I day after 5-FU dose) prote<:tion WIIS demoustrated regarding nertlropenia, bon~ marrow cytology and histopathology. lncompletC' protectiolt was revealed in mice recei1 1 ed GSH prefreatment or G-CSF prelreatment and killed I week after 5-FU dose (groups 5b and 6b respecti!Jely), while grm~ps la oud 7b give11 combiued GSH aud G-CSF pretreatment revealed uo protection .
Abstract:Background: Pramipexole is one of a new generation of dopamine agonists. Recently there have been questions regarding its neuroprotective effects. These effects have been tested against various insults, which have yielded conflicting results. Methods: In this study, we introduced a combination of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)/paraquat to induce a severe Parkinson's disease model. The mice, after receiving the combination of toxins, were evaluated using mortality rates and immunohistochemistry for degenerating tyrosine hydroxylase-positive neurons. Results and conclusions: Pramipexole was tested for its capacity to offer protection against neurotoxic the effects of MPTP/paraquat in this model; however, the results showed no improvement with pramipexole therapy.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.