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Yokokawa and IX71 Olvmpus) equipped "ith 100x objectise lens, EMCCD OXon DV897 Ander) and partide tracking software we succegsfu11) ob semed the comet 1ike behavior of EB1 GFP et vtio and in-vo Also ue apphed these fluoresce]Le imaging techniquLs to assess the effecl of anti cancer drug Thxol which known as a MT dcpolymerizeltion inhibitor on the MT growmg endg in tumor eells The medn velocity of EB1 GFP comets wag about 400 nmls at 30 OC tn-tFv and decreased by mcredsing concentration oi

This method is useful in the observation and evaluation of durability failure in old concrete specimens.

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