Background-Carbon monoxide (CO) is postulated to protect tissues against several types of injuries. We investigated the role of CO in amelioration of cardiac ischemia-reperfusion injury in vivo and the mechanisms involved in it. Methods and Results-Rats inhaled CO (250 ppm, 500 ppm, or 1000 ppm) for 24 hours in a chamber after myocardial ischemia-reperfusion induced by occluding the left anterior descending coronary artery for 30 minutes. Pre-exposure to 1000 ppm of CO significantly reduced the ratio of infarct areas to risk areas and suppressed the migration of macrophages and monocytes into infarct areas, and the expression of tumor necrosis factor (TNF)-␣ in the heart; however, 250 ppm, 500 ppm of CO, or low barometric pressure hypoxia (0.5 atm) did not affect them. Exposure to 1000 ppm CO resulted in the activation of p38 mitogen-activated protein kinase (p38MAPK), protein kinase B␣(Akt), endothelial nitric oxide synthase (eNOS), and cyclic guanosine monophosphate (cGMP) in the myocardium. Inhibition of p38MAPK, PI3kinase, NO, and soluble guanylate cyclase with SB203580, wortmannin, N(G)-nitro-L-arginine methyl ester (L-NAME), and methylene blue, respectively, attenuated the cytoprotection by CO. Conclusion-CO has beneficial effects on cardiac ischemia-reperfusion injury; this effect is mediated by p38MAPK pathway and Akt-eNOS pathway, including production of cGMP.

Our data indicate that the alanine variant of signal peptide increases the mitochondrial MnSOD activity, protects macrophages against the oxLDL-induced apoptosis, and reduces the risk of CAD and AMI.

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A 59-year-old Japanese man was referred to our hospital in March 1998 due to an acute onset of upper abdominal pain, which had occurred a few hours after ingesting raw mackerel. The physical examination showed epigastric tenderness. Upper endoscopy identified a reddish tumor, 65 mm in its largest diameter, in the gastric fundus (Figure 1). The tumor had a smooth surface, with irregular and shallow ulcers covered by blood coagula. The patients abdominal pain subsided immediately, without any treatment. An upper endoscopic examination carried out two weeks later revealed that the tumor had regressed (Figure 2). At this time, the patients serum IgGI-A antibody titer to Anisakis larvae increased to 2.09 (normal: < 1.50). On the basis of these findings, a diagnosis of gastric anisakiasis was established. The patient has been free of any gastrointestinal symptoms during a subsequent follow-up period of 26 months.