Objective This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound. Materials and Methods An experimental study with post-test only control group design was conducted. Twenty male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical analysis for the expressions of TNF-α and NF-κB. Results The highest expression of TNF-α was observed in the control group (13.20 ± 1.79), while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile the comparison between treatment groups did not highlight any significant difference (p > 0.05). The highest expression of NF-κB was observed in the control group (13.20 ± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14). NF-κB was significantly lower in the treatment group using MASE 50% when compared with other treatment groups (p < 0.05). Conclusion Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.

Purpose: To investigate the effect of mauli banana (Musa acuminate) stem topical gel extract application on the expression of transforming growth factor - β (TGF - β) and fibroblast growth factor-β (FGF-β) during the healing process in traumatic oral ulcers. Methods: The work represented a true experimental study incorporating a post test - only control group design. Four groups of male Wistar rats (Rattus norvegivus) (n = 20) with traumatic oral ulcers were given mauli banana stem extract gel of varying concentrations the negative control group: 0 %; treatment group I: 25 %; treatment group II: 37.5 %; and treatment group III: 50 %. The animals were subsequently sacrificed prior to conducting biopsy on day 5. Immunohistochemical staining was performed in order to analyze the degree of FGF-β and TGF–β expressions. Results: TGF–β was strongly expressed in treatment group II (16.80 ± 1.30). TGF-β expression was significantly different, except between treatment groups II and III (Table 2). FGF-β was strongly expressed in treatment group II (15.60 ± 3.97). There was significant difference in FGF-β expression between all the groups with the exception of treatment groups I and III. Conclusion: Topical application of mauli banana stem extract gel (37.5 % concentration) stimulates FGF-β and TGF-β expression on day 5 of traumatic oral ulcer healing process. Thus, the extract gel has potentials for clinical application for the therapy of traumatic oral ulcers

No abstract

Introduction: The approximate prevalence of Recurrent Aphthous Stomatitis (RAS) was 45.42% at Gusti Hasan Aman Dental Hospital Banjarmasin. Bacillus sp. is a bacterium that can form spores that can be transmitted through air, dust, water and food. Poor oral conditions will increase the possibility of bacterial contamination, especially for ulcers found in RAS. This study aimed to analyzed correlation between the presence of Bacillus species and OHI-S in the oral ulceration of RAS patients. Methods: This was an analytical cross-sectional study conducted among Oral Medicine Department outpatients at Gusti Hasan Aman Dental Hospital, Banjarmasin, Indonesia. A total of 38 subjects were examined to assess Oral Hygiene Index Simplified and obtained bacteria samples using the swabs technique. Bacteria were then inoculated in blood agar base media and concurrently presented with gram staining. Bacteria were then identified using the catalase test, urease test, and oxidase test. Results: Bacillus species were predominantly identified in oral ulceration of RAS patients at Gusti Hasan Aman Dental Hospital Banjarmasin. Bacillus sp. (60,5%), Streptococcus sp. (34,2%), Neisseria sp. (2,6%), and Lactobacillus sp. (2,6%) were found in ulcerated RAS lesions. Spearman test resulted in p = 0.862 (p > 0.05), where no significant difference was obtained between presence of bacillus species and OHI-s in recurrent aphthous stomatitis patients. Conclusion: Bacillus species were predominantly identified in oral ulceration of RAS patients a. However, the presence of Bacillus sp. was not associated with OHI-S in RAS patients.Keywords: bacillus sp.; oral hygiene index simplified; recurrent aphthous stomatitis

Background: The second highest burden of all diseases in world population includes infectious disease with a total of 28% cases. Angular cheilitis is an infectious condition on the corner of the mouth that may initiate masticatory, facial expression and speech dysfunction due to the involvement of orbicularis oris muscle surrounding the lips. The management of angular cheilitis comprises the elimination of causative microbes using antifungal agent. Therapeutic failure reveals the disregard of any related risk factor. Purpose: To analyze the risk factors of angular cheilitis in Gusti Hasan Aman Dental Hospital year 2019. Method: This study resorted analytical descriptive design of quantitative approach using cross-sectional method. Samples were 65 dental statuses of patients in Oral Medicine Clinic of Gusti Hasan Aman Dental Hospital year 2019. Result: Univariate data analysis presented the distribution of children category aged ≤ 18 years old (75.8%), men (54.8%), not consuming vegetable and fruit behaviour (46.8%), duration of treatment 1-7 days (56.4%), and incidence of angular cheilitis (75.8%). Fisher exact test unveiled the relationship between age and angular cheilitis (p<0.05) in which age ≤ 18 years old was 4.266 fold higher to experience angular cheilitis, while the relationship between gender and angular cheilitis (p<0.05) demonstrates the predilection of male with 4.853 fold at risk than female. Conclusion: Age and gender are risk factors in the incidence of angular cheilitis at Gusti Hasan Aman Dental Hospital year 2019.  Keywords : Age, Angular Cheilitis, Behavior, Gender