The innate immune system detects viral nucleic acids and induces type I interferon (IFN) responses. The RNA-and DNA-sensing pathways converge on the protein kinase TANK-binding kinase 1 (TBK1) and the transcription factor IFN-regulatory factor 3 (IRF3). Activation of the IFN signaling pathway is known to trigger the redistribution of key signaling molecules to punctate perinuclear structures, but the mediators of this spatiotemporal regulation have yet to be defined. Here we identify butyrophilin 3A1 (BTN3A1) as a positive regulator of nucleic acid-mediated type I IFN signaling. Depletion of BTN3A1 inhibits the cytoplasmic nucleic acid-or virus-triggered activation of IFN-β production. In the resting state, BTN3A1 is constitutively associated with TBK1. Stimulation with nucleic acids induces the redistribution of the BTN3A1-TBK1 complex to the perinuclear region, where BTN3A1 mediates the interaction between TBK1 and IRF3, leading to the phosphorylation of IRF3. Furthermore, we show that microtubuleassociated protein 4 (MAP4) controls the dynein-dependent transport of BTN3A1 in response to nucleic acid stimulation, thereby identifying MAP4 as an upstream regulator of BTN3A1. Thus, the depletion of either MAP4 or BTN3A1 impairs cytosolic DNA-or RNA-mediated type I IFN responses. Our findings demonstrate a critical role for MAP4 and BTN3A1 in the spatiotemporal regulation of TBK1, a central player in the intracellular nucleic acid-sensing pathways involved in antiviral signaling.BTN3A1 | type I IFN signaling | TBK1-IRF3 axis | MAP4 | dynein

Because of the demographic and economic changes and the lifestyle diversification, the new housing types such as the Quasi-housing and urban small-size multi-unit housing are appeared in korea. The current housing use classifications are regulated in accordance with the housing divided ownership, horizontal and vertical size of housing, and the number of units. However, there is a limit to accommodate the new housing type. Therefore, this study proposed the direction of improvement of the housing use classifications through comparative analysis of foreign housing use classifications. Also, building regulations are applied according to the building use classifications. Therefore, the new housing types in building act are very urgent and important. So this study is derived the problems of the current housing use classifications and is proposed the improvement direction of it through the expert opinion survey.