Introduction: The medical profession is characterized by high requirements for implementation of activities assessed by the quality of services provided and patient satisfaction with medical care. In this regard, healthcare professionals suffer from high psychological and emotional stress. The proposed COPSOQ III questionnaire contributes to identification and prevention of psychosocial factors affecting health and well-being of medical workers. Objective: To study international requirements for the order of translation and cultural adaptation of the long version of the COPSOQ III questionnaire on assessment of psychosocial working conditions and health promotion in medical workers in the Russian health care system. Materials and methods: The questionnaire was compiled in English. It contains 160 questions and criteria for assessing psychosocial working conditions of medical workers. The original version was translated into Russian; after that, back translation and the repeated translation was done for validation purposes and cultural adaptation of the COPSOQ questionnaire in the national health system. According to the recommendations of the COPSOQ International Network for translation and cultural adaptation of the text, we followed stages of validation and coordination of the stage outcomes and the final report. Results: To assess the linguistic load of the Russian-language version of the long COPSOQ questionnaire, its testing was carried out with participation of ten general practitioners with the positive feedback received. Conclusion: The translated into Russian and adapted long version of the COPSOQ III questionnaire is valid and recommended for use in healthcare institutions of the Russian Federation.