In this study, some N′-(substituted arylmethylidene)-4-nitrobenzenesulfonohydrazide derivatives were synthesis by reacting various aldehydes and 4-nitrobenzenesulfonohydrazide. The structural characterization of the compounds was performed by IR, 1 H-NMR and TOF-MS (compounds 3b and 3e) spectroscopic data besides elementel analyses results. The antibacterial activities of these compounds were examined against some bacteria species. The compounds showed the highest activity against Pseudomonas aeruginosa ATCC 27853. Also, anti-quorum sensing activities have been determined using a biosensor bioassay with Chromobacterium violaceum CV026 and the signaling molecule Nhexanoyl-L-homoserine lactone. All the compounds were subjected for ADME predictions by computational method.