Pollution of surface waters with coliform bacteria having antibiotic and heavy metal resistance has become an increasing public health risk. For this reason, antibiotic and heavy metal resistance profiles of fecal microorganisms in surface water samples collected from two different points (Seve Dam and Konak Pond, Kilis, Turkey) were analyzed in this study. The various physicochemical parameters such as pH, Temperature, Total Dissolved Solids (TDS), Electrical Conductivity (EC) of samples were also examined. The total coliform load in each sample was ˃1100 (MPN)/100 mL. As fecal indicator microorganisms, 21 Escherichia coli strains were isolated from sampling points. Despite the high coliform contamination, the multiple antibiotic resistance (MAR) index of E. coli was recorded as 0.187. The multiple heavy metal resistance (MHMR) index (0.202) was remarkably higher than the MAR value. Most isolates showed minimal inhibition concentrations (MIC) in the range of 25-1.600 μg/mL based on concentrations of heavy metals. These results indicate that surface waters may be discharge points of industrial and domestic wastewaters