Mycorrhizal symbiosis increases mineral nutrient uptake, especially when nitrogen applies to the fields together with biochar. To evaluate the influence of the biochar application and different levels of nitrogen on nitrogen use efficiency (NUE), yield and yield components of wheat associated with arbuscular mycorrhizal fungi (AMF) A factorial experiment with in randomized complete block design with four replications was implemented for two years (2016-2018). The experimental factors included nitrogen at four levels (urea source with 46% nitrogen) 0, 50, 100 and 150 kg N/ha, Rhizophagus Irregularis inoculum at two levels (without and with application), and biochar at three levels 0, 4 and 8 ton/ha. The results demonstrated that plant inoculated with mycorrhiza inoculum combined with 4 ton/ha of biochar showed a significant increase in, root colonization (8%). The application of will be This study proves that application of 100 kg N/ha with an amount of 4 ton/ha of biochar and AMF inoculationhas a useful and effective role in the enhancement of growth and yieldof wheat.