Prethrombin-2 (PT2) is a thrombin precursor that structurally has one glycosylation site and four disulphide bonds. As one of the fibrin glue components, thrombin is able to stick and to cover the wound. So, Thrombin can be potentially applied in replacing suture technic (Spotnitz and Prabhu 2005). Eventhough the fibrin glue has widespread applications, commercial fibrin glue has several disadvantages, i.e. allergic effect; pathogenic contamination, since the use of any plasma-derived product in the surgical setting carries a potential risk of viral transmission; and expensive protein therapeutics since must be imported (Spotnitz 2001; Enus et al. 2011). Generally, glycosylated protein therapeutics are produced from mammalian cells. Chinese Hamster Ovary (CHO) is the most commonly used mammalian