Human-induced environmental stress can impair freshwater ecosystems by destroying them or altering them in negativeways. Laws and ordinances that stress Kenya's water resources' correct management and utilization were prompted by worries about the management of freshwater bodies. Therefore, understanding of the health status of aquatic environments, particularly their biodiversity, is crucial in order to comprehend the status of water quality and limit the rate of pollution in our streams and rivers. Several well-known biological markers of water quality can be used for this. Bio-indicators are species or groups of species that can quickly reveal the abiotic or biotic condition of an environment, show how environmental change has affected a habitat or ecosystem. In many industrialized nations, including those in Europe and North America, macroinvertebrates are widely utilized as bio-indicators and are part of their national and technical standards for water quality monitoring. Their use is still relatively restricted in developing nations like Kenya. Furthermore, the use of aquatic macroinvertebrates as bioindicators of water quality to assess the condition of aquatic ecosystems is not stressed by Kenyan environmental laws and regulatory bodies. This can be because the nation doesn't have a well-known and established biomonitoring system. However, only a small number of studies have begun utilizing macroinvertebrate species as bio-indicators at this time. A greater comprehension of the macroinvertebrate variety of the Omubira River could help in the stream management due to continued municipal and agricultural activities along this river. As a result, this study examined the use of macroinvertebrates as bio-indicators of river water quality and determined how they reacted to water quality in the various region of the stream. Macroinvertebrates and selected physicochemical variables were sampled monthly in four sites for a period of four months. On each sampling sites at every sampling episode physicochemical variables including temperature, conductivity, salinity, turbidity, pH, dissolved oxygen and percentage saturation oxygen were measured using a Hydrolab Quanta Multi-Probe Meter. A Surber sampler of an area 1200 cm 2 and corer sampler were used with catching area of 20.83 cm 2 to capture macroinvertebrates. There was a general decrease of Temprature, turbidity, salinity, conductivity and pH from the town area to the forested area. However, dissolved oxygen increased from the town area to forested area. A general variation of macroinvertebrates species from manucipal area to forested area in the river was observed. The highest abundance of Belostomatidae (16.87), Chironomidae (16.77), Naididae (14.07) and Gomphidae (18.67) were found in the manucipal reach of the river. The highest abundance of Elmidae (20.49) and Notonectidae (19.87) were found in the agricultural reach while the highest abundance of Gerridae (10.53), Hydropsychidae (28.65) and Perlidae (33.92) were found at the forested reach. From the diver...