3 4 Introduction 3 5Gallstone disease (Cholelithiasis) affects 10 to 15% of the population of developed countries. Our 3 6 study aimed to determine the risk factors associated to different types of gallstone in patients 3 7 diagnosed in Khartoum State Ibnsina Gastroenterology Center. 3 8 Methods 3 9 A facility-based prospective cross-sectional study was implemented on a convenient sample of 47 4 0 participants diagnosed with gallstone through ultrasonography in Ibnsina Gastroenterology center 4 1 and who underwent surgical interventions for gallstone removal. A standardized interviewer-4 2 administrated research tool comprising three parts was used to collect data related to the 4 3 characteristics of the participants, their medical presentation and examination as well as 4 4 information on types of gallstone, surgical interventions and outcomes. The data were 4 5 computerized through Epi-info 7 and analyzed through SPSS 23. Descriptive statistics were firstly 4 6 performed and association was tested through Chi square tests and ANOVA. A multinomial 4 7 regression analysis established the relationship between types of gallstone and their associated risk 4 8 factors. All statistical tests were considered as significant when p < 0.05. 4 9 Results 5 0The risk factors statistically associated to gallstone types were family history (p = 0.011) and 5 1 duration of living in the residence area (p= 0.043) in pigment-cholesterol gallstone model vs 5 2 mixed-cholesterol gallstone model. Other four risk factors contributing to the pathogenesis of 5 3 gallstone were parity (OR = 1.623 [95% CI: 0.795-3.315]) vs (OR = 1.426, [95% CI: 0.729-5 4 2.790]), waist circumference (OR= 1.014 [95% CI: 0.948-1.085]) vs (OR = 1.001 [95% CI: 0.942-5 5 1.065]), chronic disease (OR = 0.698, [95% CI: 0.028 -17.445]) vs (OR = 0.354, [95% CI: 0.021-5 6 6.087]) and serum triglyceride (OR = 0.985, [95% CI: 0.950-1.022]) vs (OR= 0.980, [95% CI: 5 7 0.949-1.012]). 5 8 Conclusion 5 9 Our finding indicated six risk factors related to types of gallstone. Further multicenter research in 6 0Sudan on risk factors is needed to calibrate and validate our model. 6 1 Keyword: Cholelithiasis, Gallstone disease, Risk factors, Types of gallstone, Ultrasonography.6 2 6 3 9 1gallstone as well as between types of gallstone and positive history of type 2 diabetes (p=0.035). 2The other risk factors assessed were family history of GSD, history of dyslipedemia, total 9 3 cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol, 9 4 fasting blood glucose, parity and use of exogenous oestrogen in female patients, smoking and 9 5 alcohol consumption in males were not statistically associated (p >0.05) to the types of gallstone. 6Another study [29] on a sample of 100 patients investigated the association between gallstone 9 7 characteristics and 23 patient parameters. A statistically significant association was found between 9 8 both mean diastolic (p=0.012) and systolic blood pressure and types of gallstone (p=0.027).