This study aimed to demonstrate how different fish weights and clove powder concentration levels together influence anesthesia and recovery time in Cyprinus carpio. Two different body weights classes of common carp (45±5g and 95±5g) were subjected to three concentrations of clove powder (200, 300 and 400mg/L) each replicated four times. At each treatment combination, the anesthetic induction and recovery times were recorded, fish held for a period of one week to two months following the experiments to assess short-term and long-term mortality. Significant differences (P<0.05) appeared in the effect of combination between the different levels of clove powder (mg/L) with the two different weight common carp (C. carpio L.) on anesthesia time, but there was no difference in recovery time between the two size groups. The induction time was less than one minute for doses of 300 and 400 mg/L in lower fish weight 45±5g while in 200mg/L dose with 95±5g of fish weight it needed more than 9 minutes. At all tested concentrations, induction time was significantly weight-dependent (p< 0.05), but the recovery time was not. Significant alterations were recorded in the hematological variables of the fish (RBC, HB, MCH and WBC), no mortality was observed. This study has demonstrated that clove powder can be safely and effectively use in the anesthesia of common carp and anesthesia time affect by doses of clove powder with fish weight.

This study aimed to evaluate the effect of repeated freeze-thaw cycles on some electrolytes’ concentration (Na+, K+, Ca+ and Cl−) and thyroid hormones (T3 and T4), in the serum of Karadi sheep breed. This study was conducted on 18 male and female sheep (aged over one year) in two lines of Karadi sheep Jeshana and Jaff in Sulaimaniyah province. Blood samples were collected from each animal and allowed to clot for 45 to 60 min, then centrifuged at 3000 × g for 10 min to separate the serum. The serum sample was frozen at (-40) degrees and was monitored for 18 months. Serum sample analyzed after (18 months) of storage. Our result showed that repeated freeze-thaw cycling has significant and relevant increases of serum T4 (Thyroxine hormone) in both male and female groups without affecting T3 (Triiodothyronine hormone). Na+ and Cl- in both male and female groups showed a significant difference compared to the control group while the remaining electrolytes K+ and Ca+ didn’t show any relevant changes. This study has demonstrated that repeated freeze-thaw cycles do not cause changes in some biochemical constituents studied in sheep serum.

Contamination of animal feeds with mycotoxins in livestock farms is a severe matter. This study aimed to evaluate mycotoxigenic fungi, type of toxins, and toxin concentrations and contamination levels of Aflatoxin, Ochratoxin, and T2-toxin in ruminant feed collected from three different sites (a department laboratory, field 1, and field 2) and two periods of time (January and March). Also, four different genera were isolated and diagnosed from the animal feed based on the macroscopic morphology as well as on microscopic characteristics. The genera were Aspergillus spp., Penicillium spp., Fusarium spp., and Rhizopus spp. The result shows significant differences in fungal count among location storages, and among period times, too. Significant differences (P<0.05) also has found for mycotoxin concentration while storage in different locations. Additionally, animal feed collected from different sites and different periods contained lower limits of mycotoxins than the standard limits.

The recent research was managed to make an inquiry about influencing dietary fructo-oligosaccharide (FOS) on providing food supplements and growing procedure of common carp Cyprinus carpio fingerlings. The fingerlings were adapted for 3 weeks and then reared in triplicate groups in 15 tanks (n = 4 fish per tank with average initial weights of (40 ± 3.00 gm). The fish fed on experimental diets containing different levels of fructo-oligosaccharide (FOS); (0, 2.5, 5, 7.5, 10 gm FOS /kg diet) for apparent satiation twice a day for 10 weeks. Results showed that final weight, weight gain, daily growth rate, relative growth rate, and specific growth rate were significantly (P<0.05) increased with 10 gm FOS /kg diet compared to the other groups. While, food efficiency ratio and protein efficiency ratio were significantly higher (P<0.05) especially in group fed 10 gm FOS /kg diet (1.13 and 295.16) respectively compared to the other treated and control group. However, food conversion ratio in 0 gm FOS /kg diet (control) group was higher significantly (P<0.05) among other groups (1.63).