The eco-friendly and naturally source for synthesis of nanoparticles is the safe and more important methods for biosynthesis of silver nanoparticles(Ag NPS), so the goals of these study was to investigate the role of pomegranate peel extract as reducing agent for Ag+ and biosynthesis for Ag NPS and determined it is activity in vitro as antifungal. The changes in the color solution to dark brown after three days is the first step to determine the pomegranate silver nitrate nanoparticles (P-AgNO3 NPS), The UV-spectroscopy results show a broad wavelength arranged between 400-450 nm, pomegranate peel extract(PPE) was considered as reducing agent in a spectral analysis using Fourier Transformation Infrared Spectroscopy (FTIR). The average size of P-AgNO3 NPS is about 35-77 nm which determined by the scan electron microscop(SEM) and the zeta potential was -29Mv, Further the antifungal activity of P-AgNPs was evaluated at 150 ppm against Aspergillus flavus. The conclusion of these study was that the AgNPs may be synthesized quickly, easily, and cheaply using green synthesis which uses bioactive compound from natural source as reducing or stabilizing agents.

Contaminants are one of the biggest determinants of fish farming, and Cuso4 is considered one of the most important factors of pollution on microorganisms. Probiotics are among the most important feed additives and have a major role in improving the condition of fish. Yeasts, especially Saccharomyces cerevisiae, are considered one of the best additives that have a role in reducing the effect of copper on fish. Therefore, our study was done in adding yeast to the diet to reduce the effect of Cuso4, and This experiment was conducted in the fish laboratory of the Department of Animal Resources / College of Agriculture and Forestry / University of Mosul, where fish were brought from Erbil Governorate / Tarjan hatchery in ideal transport conditions and the fish were acclimatized in Ponds outside the laboratory for a week, after which the fish were sterilized with salt, and then the fish were transferred to the laboratory and after randomly distributing them with a weight of 35+-2 with 7 fish for each pond by 12 ponds every 3 treated ponds. The first treatment included the second control, the third yeast Cuso4, the fourth yeast and cuso4 and continued The experiment lasted for 56 days, and after the end of the experiment, it was noted that the yeast had improved the weight and growth parameters in general, and the yeast had reduced the effect of Cuso4 by the fourth treatment.