Background: Vitamin D deficiency is one of the most common nutritional deficiencies. Cardiovascular disease patients are also prone to this condition. Recently, a relationship between vitamin D deficiency and cardiovascular diseases has been suggested. This study aims to compare the relationship between ventricular systolic function and vitamin D deficiency. Methods: This study investigated patients without obvious coronary artery disease between 2020 and 2021. First, vitamin D levels were measured in the patients. Then, they were divided into two groups based on a 30 ng/dl cut-off point. All patients underwent echocardiography and ventricular systolic function parameters were evaluated and compared. Results: In this study, 27 patients with normal vitamin D levels and 47 patients with vitamin D deficiency entered the study. There was no significant difference in demographic variables and underlying diseases between these two groups. There was no significant difference between left ventricular (LV) systolic function parameters including ejection fraction (EF), and LV end-systolic/diastolic volume. No significant difference was also observed between right ventricular (RV) systolic function parameters including Tricuspid Annular Plane Systolic Excursion (TAPSE), RV fractional area change (RVFAC), Right ventricular systolic velocity (RVSM) in tissue Doppler echocardiography as well as RV diastolic parameters such as A, E, E´, deceleration time (DT), right atrial volume (RAVi) as a precursor of right ventricular systolic dysfunction groups. Conclusion: Based on the results of this study, there is no relationship between vitamin D levels and ventricular systolic dysfunction.