Introduction: Cumin, due to its food and medicinal properties, is one of the important plant species in the world. Moreover, water and nutrition deficiencies are serious abiotic stress factors. So, this experiment was conducted with the aim of investigating the effects of sulfur and foliar application of Fe and Zn on yield and biochemical characteristics of cumin under irrigation regimes. Methods: The experiment was conducted as a split plot on the basis of a completely randomized block design during the 2016-2017 growing seasons with three replications. Experimental factors were arranged in irrigation regimes as main plots at three levels (I1: No stress (control), I2: irrigation based on 40% available water discharge, I3: 80% available water discharge) and foliar application of Zinc (Zn) and Iron (Fe) as subplots [F1: control (water-soluble), F2: Iron, F3: Zinc, F4: Zinc and Iron chelate] and sub-sub plots including sulfur fertilizer [S1: control (no use of sulfur), S2: sulfur fertilizer with Thiobacillus]. Results: Analyzed data showed that total phenol content and flavonoids were enhanced with the increase of drought intensity and the maximum amount was recorded under I3, while I3 caused a substantial reduction in grain yield. Flavonoid and grain yield significantly increased in F4. Total phenol content was the highest in F2 and F3 treatments. Application of sulfur fertilizer resulted in a significant increase in peroxidase, phenol and flavonoids. The highest amount of peroxidase was obtained in I3F4 and I3F3. The largest total soluble sugar (TSS) was resulted by I3S2 and the least by I1S1. Foliar application of Zn and Fe with sulfur fertilizer increased TSS. Conclusion: The present study suggests that foliar application of Fe and Zn and sulfur fertilizer can improve the injurious effects of water deficiency on cumin plant through alteration in yield and biochemical characteristics.