Background: Searching the biomarker from complex heterogeneous material for early detection of disease is a challenging task in the field of biomedical sciences. Objective: The study has been arranged to explore the proteomics serum derived profiling of the differential expressed and low molecular weight protein in breast cancer patient. Method: Quantitative proteome was analyzed using the Nano LC/Mass and Bioinformatics tool. Results: This quantification yields 239 total protein constituting 29% of differentially expressed protein, with 82% downregulated differential protein and 18% up-regulated differential protein. While 12% of total protein were found to be cancer inducing proteins. Gene Ontology (GO) described that the altered proteins with 0-60 kDa mass in nucleus, cytosol, ER, and mitochondria were abundant that chiefly controlled the RNA, DNA, ATP, Ca ion and receptor bindings Conclusion: The study demonstrate that the organelle specific, low molecular weighted proteins are significantly important biomarker. That act as strong agents in the prognosis.

The nanotechnological arena has revolutionized the diagnostic efficacies by investigating the protein corona. This displays provoking proficiencies in determining biomarkers and diagnostic fingerprints for early detection and advanced therapeutics. The green synthesized iron oxide nanoparticles were prepared via Withania coagulans and were well characterized using UV–visible spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and nano-LC mass spectrophotometry. Iron oxides were rod-shaped with an average size of 17.32 nm and have crystalline properties. The as-synthesized nanotool mediated firm nano biointeraction with the proteins in treatment with nine different cancers. The resultant of the proteome series was filtered oddly that highlighted the variant proteins within the differentially expressed proteins on behalf of nano-bioinformatics. Further magnification focused on S13_N, RS15, RAB, and 14_3_3 domains and few abundant motifs that aid scanning biomarkers. The entire set of variant proteins contracting to common proteins elucidates the underlining mechanical proteins that are marginally assessed using the robotic nanotechnology. Additionally, the iron rods indirectly possess a prognostic effect in manipulating expression of proteins through a smarter route. Thereby, such biologically designed nanotools provide a dual approach for medical studies.

In the current era, cancer is a critical health problem. Purpose of the study was symptomatic analysis of cancer patients and medical evaluation of hematological and biochemical parameters of patients from different geographical areas of district Bahawalpur. A crosssectional study was done to find out the diagnostic timeliness in cancer patients. Out of 150 cancer patients, 53% patients were males and 47% females with  age from 15 years to 75 years. It has been shown that 76% of patients represent major symptoms of cancer while 24% of patients were showing minor symptoms. Furthermore, cancer patients were disturbed metabolical, hematological, and biochemical profiling. There were 83% of patients with anemia and 17% patients have normal hemoglobin count in males and 85 % of total female patients have low hemoglobin level while rest of 15% have normal hemoglobin count. Illiteracy is positively influencing the factors responsible for causing cancer with 53%, whereas 23% less than primary level education and only 1% of patients had completed their graduation. There were 31% of the patients lies in upper lower class of society, 28% lower class, Lower middle class 19%, middle class 14%, upper middle class 7% and only 1 % patients belong to high social status. This study will help to diagnosis the early cancer detection via biochemical and metabolic profiling.