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-The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies.

Vermicomposting is at suitable methods for stabilization and converting of sewage sludge into useful products. To investigate the effects of bulking material on vermicompost quality, an experiment was conducted in a factorial design with three replications. In this experiment, the final dried sludge in lagoons was mixed with bulking materials (woodchips, three leaves, and wheat straw) in four mixing proportion with swage sludge (0, 15, 30 and 45%, V/V). Chemical properties of bulking materials, sewage sludge, and produced vermicomposts were determined. Vermicomposts had relatively low pH and electrical conductivity (EC). The nutrients content of vermicompost, especially N, P, Fe, and Zn, were high. These properties showed its suitable quality for use in agriculture. Heavy metals concentrations of vermicomposts, except Zn, were lower than recommended values by EPA. Mixing of Bulking materials with sewage sludge decreased pH, N, Pb, Co, and Cd, but increased Ca, Mg, Fe, Cu, Mn, Zn, and Ni content of produced vermicomposts. In most cases, the concentrations of nutrients were higher in 30% mixing proportion. Therefore, 30% (v/v) mixing of bulking material with sewage sludge is recommended for vermicompost production.Index terms: Vermicompost, sewage sludge, bulking materials, heavy metals, Eisenia foetid. RESUMOA vermicompostagem é um métodos adequados para a estabilização e conversão de lodo de esgoto em produtos úteis. Para investigar o efeito de aumentar o volume de material sobre a qualidade do vermicomposto, foi conduzido um experimento em esquema fatorial com três repetições. Neste experimento, o lodo final seco em lagoas foi misturado com materiais de volume (lascas de madeira, três folhas e palha de trigo) em quatro proporções de mistura (0, 15, 30 e 45% V / V) com lodo de esgoto. Propriedades químicas dos materiais de amontoamento, lodo de esgoto e vermicompostos produzidos foram determinados. Vermicomposto apresentaram pH relativamente baixo e CE. O seu teor de nutrientes, especialmente N, P, Fe e Zn foram elevados. Essas propriedades mostrou sua qualidade adequada para utilização na agricultura. Concentrações de metais pesados de vermicompostos, com exceção do zinco, foram menores do que os valores recomendados pela EPA. A mistura de materiais Bulking com lodo de esgoto diminuiu o pH, N, Pb, Co e Cd, mas aumentou a concentração de Ca, Mg, Fe, Mn, Cu, Zn, Ni no vermicomposto produzido. Na maioria dos casos, as concentrações de nutrientes foram maiores na proporção de 30% de mistura Portanto, recomenda-se a mistura de materiais ao lodo na proporção de 30% para a produção de vermicomposto.Termos para indexação: Vermicomposto, lodo de esgoto, materiais de volume, metais pesados, Eisenia foetid.

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