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Aim: To evaluate the in-vivo antioxidant activity of chloroform extract fractions of Indigofera barberi (whole plant) against paracetamol induced toxicity in rats. Methods: For 7 days, animals were treated with chloroform extract fractions and the toxicity was induced with a single dose of paracetamol intra peritoneal injection. Group of animals pretreated with 100 mg/kg p.o of fraction D of Indigofera barberi improved the SOD, catalase, peroxidase and glutathione levels significantly as compared to control group. Results: The results showed that the activities of SOD, catalase, peroxidase and glutathione in group treated with paracetamol are 33.6 ± 0.09, 5.5 ± 0.23, 0.131 ± 0.15 & 46.1 ± 5.81 and SOD, catalase, peroxidase & glutathione in fraction D treated group are 61.8 ± 0.07, 10.6 ± 0.16, 0.913 ± 0.23 & 87.6 ± 1.4 respectively. So, the present study revealed that fraction D of Indigofera barberi has significant in-vivo antioxidant activity and can be used to protect tissue from oxidative stress. Conclusion: From the results, fraction D of Indigofera barberi in the dose of 100 mg/kg, p.o. has improved the SOD, catalase, peroxidase and glutathione levels significantly. Based on this u n c o r r e c t e d p r o o f study we conclude that fraction D of Indigofera barberi possesses in-vivo antioxidant activity and can be employed in protecting tissue from oxidative stress.

Background: Michelia nilagirica belonging to the family Mangoliaceae is commonly used by many traditional healers in most of the herbal preparations for diabetes and kidney diseases. Objective: Different fractions isolated from ethanolic extract of whole plant of Michelia nilagirica is investigated for hepatoprotective activity in wistar albino rats against paracetamol induced hepatic injury. Materials & Methods: Rats were divided into eight groups. Each group contains six animals. Hepatic injury was achieved by injecting paracetamol at a dose of 2 mg/kg p.o. Results: The hepatoprotective action is seen with fraction A by reduction in serum marker enzymes like Aspartate transaminase (AST), Alanine transaminase (ALT). It also reduced the elevated levels of Alkaline phosphotase (ALP) & Serum bilirubin. Conclusion: Histopathological studies further confined the hepatoprotective activity of fraction A against paracetamol treated group. The results obtained were compared with silymarin (100 mg/kg, orally), a standard drug.