In this paper, the design, simulation, and fabrication of a novel printed rectangular slot antenna with a band-notched function suitable for 2.4 GHz wireless local area network (WLAN) and ultra-wideband (UWB) applications is presented and investigated. Two pairs of slits are introduced into the ground plane to realize band-notched function, by tuning the position, length, and width of which a suitable rejected frequency band can be obtained. To improve the impedance matching, a rectangular cut is also made in the ground plane so that the antenna can cover 2-12 GHz frequency range. According to the measured results, the proposed antenna has a large bandwidth totally satisfying the requirement of 2.4 GHz WLAN and UWB systems, while providing the required band-notch function from 5.1 to 5.9 GHz. The study of transfer function and time-domain characteristics also indicates the band-notched function of the antenna. The radiation patterns display nearly omni-directional performance and the antenna gain is stable except in the rejected frequency band (5.1-5.9 GHz). Moreover, group delays are within 1.5 ns except for the notch band. These features make it a promising candidate for UWB wireless applications. Details of this antenna are described, and the experimental results of the constructed prototype are given.