The recent study was conducted on University of Basrah College of nursing and aimed to assess the nursing knowledge regarding myocardial infarction. Fifty nurses from both gender (male and female) with different educational level and experience as well as work field participate the assessment which include the demographic information and items corresponding myocardial infarction. The result showed that 68 % of the participants female, and most of them with diplomat degree, nurses have 1-5 year of work experience were (50%), also the results showed that their knowledge (correct answer extend from 10-78%) with significant total mean of score (2.2). The study concluded the participant’s nurses have a knowledge regarding myocardial infarction but the scientific support for this disease is important to raise the scientific and professional competence of nurse.