Schistosomiasis is a chronic and debilitating disease especially in developing countries. S. haematobium of the urinogenital system is a major human health problem across Africa. Genetic variability recommended studying variation in disease burden and pathology, and epidemiological aspects. The present study investigated genetic diversity and polymorphism human S. S. haematobium isolated by using RAPD-PCR technique on 50/100 urine samples from different areas in Sohag Governorate.Extracted DNA was used for molecular analysis using RAPD-PCR primers (A01, A02, A12, A13, & Y20). 27 S. haematobium DNA isolates (54%) were amplified by using specific primers. The isolates showed distinct variation in size and number of amplified fragments, indicating high genetic variation among them.