Zanthoxylum armatum DC., belonged to the family Ruteacea, is a medicinal plant used to cure many diseases. DNA barcoding was used as a tool for molecular identification of Zanthoxylum armatum DC. species from Balakot Pakistan. In the present study four DNA barcodes including matk, rbcl, ITS and trnH-psbA were used. The sequenced data were analyzed by using BLASTn at NCBI, FASTA and Mega 7.0 software. During PCR analysis, 3 DNA barcodes ITS, rbcl and trnh-psbA were successfully amplified and showed the 100% sequencing success. Furthermore, these barcode markers showed 99-100% sequence similarity with the reference sequences at the BLASTn. The further analysis revealed the sequence similarity of investigating marker with Zanthoxylum armatum (MH016484.1), Zanthoxylum nitidum (FN599471.1) and Zanthoxylum bungeanum (MF097123.1) respectively. The current finding provides the basis for sequenced data of Z. armatum to be used in future for molecular discrimination among the plant species from Pakistan and it is concluded that combination of diverse kind of barcoding markers could be helpful in proper identification of species at lower taxonomic level. Bangladesh J. Plant Taxon. 27(2): 323-333, 2020 (December)

C itrus sinensis L. commonly known as sweet orange, part of the citrus group, belongs to Rutaceae family. Citrus Sinensis is originated from northern China and is adoptive to tropical, subtropical and mild temperate climates (Piccinelli et al., 2008). Citrus fruits are the most important and widely grown non-climacteric fruit crops (Paul et al., 2012). Temperature ranges from 30 to 40 °C is found to be adequate for tree growth and yield. Citrus fruits are less perishable fruit. The respiration rate which is an important element of the shelf life of fruit is affected by humidity, temperature, movement of air, bruises, and microbial activity (Chhikara et al., 2018).Citrus sinensis is a very important fruit and export commodity of Pakistan a total production of 2334 thousand tons on an area of 195,000 ha (Swinnen and Squicciarini, 2012;Jabeen et al., 2016). In Pakistan major producing areas are Punjab and Khyber Pakhtunkhwa (Peshawar, Malakand, Dir, Buner, Nowshera and Haripur). In the Khyber Pakhtunkhwa province, the major citrus grown is sweet orange having 32.30 thousand tons per annum production from 4000 hectares (Ghafoor et al., 2016).