This
study investigated the physicochemical characteristics and
antioxidative role of novel acidic cell-bound exopolysaccharide (EPS-b)
from probiotic Limosilactobacillus fermentum (MTCC 5898) and gained an insight into the structure–function
relationship. The physicochemical analysis of EPS-b isolated by ultrasonication
method revealed a heteropolysaccharide molecule with an average MW
of 96.97 kDa composed of glucose and galactose subunits present in
random-coiled conformation. Fourier transform infrared (FTIR) and
nuclear magnetic resonance (NMR) analyses further supported the observation
and indicated the presence of α-(1 → 6) linkages. The
analyses implicated the significant influence of structural features
on the antioxidative activity of EPS-b by showing remarkable ABTS
scavenging, reducing, and metal chelating potential with increasing
concentration. Besides, the EPS-b by its scavenging potential also
maintained the oxidative balance in the Caco-2 cells under oxidative
stress and preserved the cellular antioxidative defense system (CAT,
GPx, SOD, HO1, and GCLC) at the
basal level.
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