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Methionine and other sulfides were rapidly and efficiently oxidized to their corresponding sulfoxides at neutral pH in the presence of Mn2+, oxygen, and sulfite ion. The sulfoxide formation was dependent on the aerobic oxidation of sulfite. In the presence of excess methionine, more than 0.5 mole of methionine was oxidized to sulfoxide per mole of sulfite oxidized. Superoxide dismutase inhibited sulfite oxidation and thus inhibited sulfoxide formation. Other sulfides, such as S-methylcysteine, were not appreciably

Five hours after cut carnations had been treated with a pulse of 1 or 4 mM silver thiosulfate (STS), in vivo ethylene binding in petals was inhibited by 22 and 29%, respectively . When binding was measured 4 days after the 4-mM STS treatment, binding was inhibited by 81% . 2,5-Norbornadiene, which substantially delays carnation senescence, inhibited ethylene binding by 41% at a concentration of l000µ1/1. The Kd for ethylene binding in carnations was estimated to be O .lpl/l in petals and 0 .O9µ1/1 in leaves. The concentration of binding sites was estimated to be 6.0 X 10 -' mol/kg of petals and 2 .0 X 10 -' mol/kg of leaves

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