Two types of loading protocol, i.e., monotonic loading and cyclic loading, were compared to investigate the effect on the mechanical properties of 316L stainless steel. The specimen used is a dog-bone specimen in accordance with ASTM E8 and ASTM E606 standard. For cyclic loading, the multiple-step method is used to obtain the hysteresis loop of the material. A total of 13 strain amplitude conditions had been carried out, ranging from 0.05%-0.65%. The stress-strain curve shows that the cyclic stress-strain curve is higher than the monotonic stress-strain curve due to the cyclic hardening behaviour in the 316L stainless steel. The cyclic hardening behaviour increases the ultimate tensile stress of the material. However, when the material gains its strength through cyclic hardening, the ductility in the material will decrease.