Ferroptosis is a novel form of cell death that plays a key role in several diseases, including inflammation and tumours; however, the role of ferroptosis‐related genes in diabetic foot remains unclear. Herein, diabetic foot‐related genes were downloaded from the Gene Expression Omnibus and the ferroptosis database (FerrDb). The least absolute shrinkage and selection operator regression algorithm was used to construct a related risk model, and differentially expressed genes were analysed through immune infiltration. Finally, we identified relevant core genes through a protein–protein interaction network, subsequently verified using immunohistochemistry. Comprehensive analysis showed 198 genes that were differentially expressed during ferroptosis. Based on functional enrichment analysis, these genes were primarily involved in cell response, chemical stimulation, and autophagy. Using the CIBERSORT algorithm, we calculated the immune infiltration of 22 different types of immune cells in diabetic foot and normal tissues. The protein–protein interaction network identified the hub gene TP53, and according to immunohistochemistry, the expression of TP53 was high in diabetic foot tissues but low in normal tissues. Accordingly, we identified the ferroptosis‐related gene TP53 in the diabetic foot, which may play a key role in the pathogenesis of diabetic foot and could be used as a potential biomarker.

Objective To analyze the clinical characteristics of patients with overweight acute type A aortic dissection, and to explore the risk factors of acute kidney injury in patients with overweight acute type A aortic dissection. Methods From March 2019 to February 2022, the clinical data of 71 patients with acute type a aortic dissection diagnosed by CTA and undergoing surgical treatment with BMI > 24 in the First People's Hospital of Yunnan Province were retrospectively analyzed, and analyzed by univariate and logistic multivariate analysis methods. Results The mean BMI of all included patients was 27.23, The mean surface area of all included human populations was 1.833. The mean age of all patients was (52.06 ± 10.71) years old, and 35 patients developed acute kidney injury after surgery. Multi-factor Logistics regression analysis confirmed the risk factors for postoperative acute kidney injury in overweight patients with acute type A aortic dissection, including gender, CPB transit time and intraoperative infusion of suspended red blood cells. Seven patients in the AKI group died in hospital after surgery and two patients died in the non-AKI group. Conclusions Among patients with overweight acute Type A aortic dissection, the incidence of AKI is 49.30%. According to multi-factor Logistics regression analysis, gender, CPB transit time and intraoperative suspended red blood cell volume are independent risk factors for postoperative acute kidney injury in patients with overweight acute Type A aortic dissection.

Objective: Acute mesenteric vein thrombosis (AMVT) is one of the acute abdominal diseases with onset, rapid progression, extensive intestinal necrosis, and requiring immediate surgical resection. The purpose of this study was to determine the risk factors of nosocomial intestinal resection in patients with AMVT. Methods: We retrospectively analyzed 64 patients with AMVT diagnosed by CTA in Affiliated Hospital of Kunming University of Science and Technology from January 2013 to December 2021. We compared patients who underwent intestinal resection (42 cases) with those who did not undergo intestinal resection (22 cases). The area under the ROC curve was evaluated and the forest map was drawn. Results: Among the 64 patients, 6 cases (9.38%) had a fever, 60 cases (93.75%) had abdominal pain, 9 cases (14.06%) had a history of diabetes, 8 cases (12.5%) had a history of deep vein thrombosis (DVT), and 25 cases (39.06%) had ascites suggested by B ultrasound or CT after admission. The mean age of all patients was (49.86±16.25) years. The mean age of patients in the enterectomy group was (47.71±16.20) years. The mean age of patients in the conservative treatment group (without enterectomy) was (53.95±15.90) years. In univariate analysis, there were statistically significant differences in leukocyte count (P=0.003), neutrophil count (P=0.001), AST(P=0.048), total bilirubin (P=0.047), fibrinogen (P= 0.022) and DD2 (P= 0.024) between the two groups. Multivariate logistic regression analysis showed that admission white blood cell count (OR=1.153, 95%CI: 1.039-1.280P =0.007) was an independent risk factor for intestinal resection in patients with AMVT. ROC curve showed that white blood cell count (AUC=0.759 95%CI: 0.620-0.897P =0.001 optimal threshold :7.815 sensitivity: 0.881 specificity: 0.636) had a good predictive value for emergency enterectomy for AMVT. Conclusions: Among patients with AMVT, patients with a higher white blood cell count at admission were more likely to have intestinal necrosis and require emergency enterectomy. This study is helpful for clinicians to accurately determine whether emergency intestinal resection is needed in patients with AMVT after admission, prevent further intestinal necrosis, and improve the prognosis of patients.