Background: Infant labial frenum morphology will directly lead to the midline diastema between maxillary deciduous tooth，which locates in the facial aesthetic area。 facial aesthetic area causes family members' medical demands. Objective: To explore the correlation of infant labial frenum morphology and maxillary deciduous tooth eruption with the age of infants and provide theoretical support for clinical practice. Methods: 1,268 infants (730 males and 538 females) of 5-36 months old who had routine physical examinations in the Children’s Health Center of Maternal and Child Health Hospital of Ningxia Hui Autonomous Region in 2022 were selected. Multiple linear regression was used to assess the correlation of labial frenum morphology with age and the correlation of maxillary deciduous tooth eruption with age. The age discrimination equation between different genders was also established. Results: There was a negative correlation between labial frenum morphology and age (male: r=-0.567, female: r=-0.571, P<0.001) and a close relation between maxillary deciduous tooth eruption and age (male: r=0.769, female: r=0.803, P<0.00). Conclusion: The correlation of labial frenum morphology and maxillary deciduous tooth eruption with age can provide guidance to the aesthetic anxiety of infants’ family members.