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Introduction and objectives: It was urgent to explain the role of egg yolk allergen sensitization to the egg allergic population and we would evaluate the diagnostic value of allergen components in whole eggs, including egg white and egg yolk.Materials and methods: Firstly, we collected 99 positive and 21 negative sera against egg allergy. Then we used modified enzyme linked immunosorbent assay (ELISA) to survey specific IgE (sIgE) to all-proven and single component in eggs, Ovomucoid (Gal d 1), Ovalbumin (Gal d 2), Ovotransferrin (Gal d 3), Lysozyme C (Gal d 4), Serum Albumin (Gal d 5), and YGP42(Gal d 6) in allergic and non-allergic populations. Last but not least, we studied the sIgE reactivities to egg allergen components by receiver operating characteristic (ROC) analysis.Results: Among egg-allergic individuals, nearly 10% were sensitized to five of six egg allergen components, and the cross-reaction frequency between two egg yolk allergens with Gal d 1 was about 30% in the groups diagnosed with egg allergy or non-allergy. The best component-combination diagnosis in egg allergy of Gal d 1+ Gal d 6 demonstrated the largest area under curve (AUC) of 0.994.Conclusions: Our results suggested that there were individual differences in allergenicity of different egg allergen components, especially in the samples negative to egg allergy diagnosed but sensitive to egg yolk components. It was indicated that component resolved diagnosis of egg yolk improved the value for egg allergy management indispensably.

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Sensitization to allergens and their peptides varies among patients due to geographical or ethnic differences. The present study aimed to investigate immunoglobulin (ig)e and igG 4 responses to tropomyosin and its peptides in shrimp allergic patients from northern china. a total of 92 subjects were studied, including 35 shrimp allergic patients, 29 patients with house dust mite (HdM) and/or cockroach allergic patients and 28 healthy volunteers. Serum ige and igG 4 antibodies to recombinant shrimp tropomyosin (rPen a 1) and its peptides were measured by means of a light-initiated chemiluminescent assay. a total of 9 major sequential epitopes of Pen a 1 reported in the literature were synthesized. of 35 shrimp allergic patients, 25 (71.4%) had positive Pen 1-specific IgE (sige) antibodies and 22 (62.9%) contained measurable rPen a 1-specific igG 4 (sigG 4 ) antibodies. a strong igG 4 response accompanied the presence of ige to Pen a 1. none of the patients with HdM and/or cockroach allergy demonstrated ige reactivity to rPen a 1. The reaction frequency of ige binding epitope was 20-48%, while that of igG 4 binding epitope was 63.6-90.9%. The ige and igG 4 recognition patterns of the tropomyosin peptides demonstrated high interpatient heterogeneity. diversity of ige binding epitopes was positively correlated with Pen a 1 sige levels. in the study population, tropomyosin was a major allergen recognized by the majority of shrimp allergic patients, which is consistent with previous reports. However, none of the 9 epitopes are major (reaction frequency >50%) ige-binding regions, indicating the epitopes profile may be different in other regions.

CO tissue is an important allergen source, and Eri s 2 is a new crab allergen. This study provides insights that will be useful for component-resolved diagnostics for crab allergy.