The present investigation was carried out during rabi, 2019-20 at Jaguli instructional farm of Bidhan Chandra Krishi Viswavidyalaya by using four varieties (ADV414, Bulet, TBM 204 and ADV 406) of rapeseed-mustard. Altogether two insects viz., mustard aphid (Lipaphis erysimi) and sawfly (Athalia lugens proxima) cause most of the damages at different crop growth stages. In the middle of January maximum intensity of sawfly was observed when the crop was flower bud formation stage. Maximum aphid population was noticed during 2 nd week of February at silica formation stage of the crop irrespective of the varieties. The aphid population was very strongly correlated with the incidence of coccinellid population. The correlation study between sawfly population and weather parameters revealed that maximum and minimum temperature had significant negative correlation with the pest population. Among the weather parameters minimum relative humidity showed significant negative correlation with the aphid and coccinellid population. Regression studies indicated that temperature and relative humidity were the most influencing factors over the incidence of sawfly and aphid population respectively.

The Study evaluates effects of Seasonal Variation on Antioxidant Potential and Total Phenolic Content of Zanthoxylum armatum. DPPH and FRAP methods were used to evaluate effect of seasonal discrepancy on the antioxidant potential of plant Zanthoxylum armatum. The investigation observed that the antioxidant activity depends upon total phenolic and flavonoid contents which may affect by seasonal discrepancy. Study observed abundant of phenolic and flavonoid during summer season resulting potent antioxidant activities. The moderate antioxidant activity was observed in monsoon season. Study concluded that total phenolic content increased from rainy to summer season and thus antioxidant activities also vary with seasonal discrepancy.

Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.Â

The study represents phytochemical standardization and antioxidant potential of ayurveda formulation Darvyadi Raskriya. The study involves development of high-performance thin-layer chromatographic (HPTLC) method for analysis of formulation. The study utilizes analysis of glycyrrhizin in formulation which is the phytoconstituent of Glycyrrhiza glabra one of the component of formulation. The sample in ethanol was applied on aluminium TLC plates using Linomat 5 spray (CAMAG). Linear ascending development was performed in twin trough glass chamber saturated with mobile phase. The mobile phase consisted of ethyl acetatemethanol-formic acid (10:5:1 v/v/v). The spectrodensitometric detection was performed at the wavelength of 254 nm. The regression analysis was found to be linear with r 2 =0.997 in the concentration range 5-25 ppm. The antioxidant activity of formulation was also found to be significant as compared to control.

Yashtimadhu Churna is one of the traditional formulations used as antacid, anti-inflammatory, anticancer, antioxidant, analgesic, anti-stress, demulcent, antibacterial and anti-asthmatic agents. The formulation mainly contains Yastimadhu (Glycyrrhiza glabra) root extract. Present study described preliminary qualitative phytochemicals analysis and chromatographic fingerprinting of Yashtimadhu Churna using HPLC and HPTLC analysis. Study also presented anti-inflammatory potential of formulation using protein denaturation method. This study helps to establish standardization parameters of Yashtimadhu Churna and also confirmed anti-inflammatory potential of formulation.