Medicinal plants are the important source of potentially useful chemotherapeutic agents which have made enormous contributions to human health and well-being. The methanolic extract of leaves of Eucalyptus tereticornis for antibacterial activity against the bacterial strain Escherichia coli was investigated. Response surface methodology (RSM) using central composite design (CCD) was adopted to optimize the effect of process variables.The optimum conditions for maximum antibacterial activity (zones of inhibition, mm) were found out to be E. tereticornis extract i.e., Limonene, 3 mL; 1, 8-Cineole, 11 mL; Terpinen-4-ol, 15 mL; pH, 7.0 and temperature, 40 C. The results suggest that the organic (methanol) extract of E. tereticornis could be a possible source to obtain new and effective herbal medicines to combat infections caused by multi-drug resistant microbial strains from community and hospital settings.

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The purpose of the experiment was to find out the impact of various combinations of growth regulators 6-Benzylaminopurine (BAP cytokinins family) and Indole-3-acetic acid (IAA auxin family) on growth and root induction of banana explants micro-propagated in vitro. Explants were obtained from young suckers of 8818-william, Pisang and Brazilian varieties. The explants were cultured in vitro containing MS media and different BAP and IAA combinations. The results showed banana varieties exhibited differences for the shoot and root development and also for the number of shoots and leaves. Wiallium-8818 hybrid gave more shoots (7.33) when the concentration of BAP and IAA was 2.5 and 1 mg L -1 , respectively. Alike, Wiallium-8818 hybrid produced the longest shoot (12.20 cm) with more leaves (6.23) at 1 mg L -1 of each BAP and IAA. But Pisang variety produced maximum fresh weight (15.45 g) with a greater number of roots at 5 + 1 mg L -1 of BAP + IAA, respectively.

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