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Human gait is performed by the locomotion of the lower limbs. The basic spatial parameters of gait cycle were measured in laboratory for the young healthy subjects including 13 females and one male in two conditions i.e. barefoot (assuming healthy gait) and wearing only one shoe (assuming unhealthy or compensated gait). The comparison between the two conditions was studied for the average values of step length, stride length, base of support and step width. Also, the standard deviation and coefficient of variance were calculated individually for each parameter of healthy subjects. The average values of the initial three parameters were greater for the healthy subjects while the fourth parameter calculated was greater for the unhealthy subjects.