Materials and Methods Materials Serratiopeptidase was obtained from Advanced Enzyme Technologies, Thane, India. Eudragit L100 was gifted by Evonik Roehm Pharma Polymer, Mumbai, India. Clove oil, Tween 80, ethanol and HPMC K4M were purchased from S.D. Fine Chemicals, Mumbai, India. All other chemicals used were of analytical grades. Methods Preparation of buccal patch Non-emulsification method: Ten mg serratiopeptidase was dissolved in ethanol at ambient temperature under continuous stirring and then added to alcoholic HPMC solution. Later, clove oil and Eudragit L100 were added to form homogenous mixture. This mixture was poured in a petri dish and dried at room temperature for 12 h [7]. The dried film was removed and stored in a desiccator. Different patches of polymer concentrations of HPMC and Eudragit L100 were prepared in various ratios 1:1, 3:2, 2:3 and 4.5:5.5 were abbreviated as F1, F2, F3 and F4, respectively as shown in Table 1.