Phalaenopsis amabilis (L.) Blume Orchid is one of the species in Phalaenopsis genus and are well-known as exotic and beautiful white flowers. Besides that, there is little continuity in the mass propagation of Phalaenopsis amabilis (L.) that produce many standardized seedlings in a relatively short period to satisfy market demand. This study was focusing on evaluating the effect of natural additives as plant growth regulators (PGR). PGR was used as nutritional constituents in the media culture to enhance the growth of explants. The additives used in this study were coconut water and peptone at different concentration, which were 5 %, 7.5 %, and 10 % of coconut water (CW) and 1 % and 2 % of peptone that supplemented in Murashige and Skoog (MS) medium. These additives were added to examine the height of plantlets, number of leaves, and number of roots after 2 months cultured. Result showed that addition of 5% CW and 2 % peptone in treatment 10 recorded the highest height of plantlet which was 1.14±0.060 cm. The highest number of leaves with 2.18±0.507 was observed in treatment 11 (7.5 % of CW and 2 % of peptone) and number of roots with 1.928±0.286 was from treatment 9 (0% of CW and 2% of peptone). Coconut water has been proved can stimulate and enhance growth and proliferation of Phalaenopsis amabilis (L.) plus the existence of peptone may help in development and increase the percentage of survival rate of Phalaenopsis amabilis (L.) due to the content of peptone which are carbon and nitrogen sources. The observation from all the treatments produces healthy explants as the natural additive can substitute the use of synthetic PGR.

Kelip Orchid (Phalaenopsis bellina) is dominantly growing in Kalimantan, Sumatera, and Malaya Peninsula; however, this orchid is dangered due to deforestation activity. To Conserve the orchid Kelip, a plant tissue culture technique can be applied. The research objective was to evaluate the growth and budding optimum time of the orchid Kelip by adding a plant growth regulator from Ambon banana extract and coconut water using in vitro method. This research used a completely random design (CRD), such as a control and nine treatments in triplicate. The treatment was used in various concentration combination of Ambon banana extract (75 mL/L; 100 mL/L; 125 mL/L) and coconut water (50 mL/L; 100 mL/L; 150 mL/L) that mixed within Murashige and Skoog medium (MS) to observe budding time and growth of Kelip Orchid. The result showed that combining Ambon banana extract and coconut water positively impacted Kelip budding growth. The optimum concentration to improve the faster budding growth was two weeks after planting (WAP), and the higher number of budding was about 5 in 10 WAP. Hence, Ambon banana extract and coconut water mixed (1:1) can be developed as natural plant growth regulators for Kelip horticulture sustainability.