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The distribution coefficients, K, for 1-butanol, 1-pentanol, 1-hexanol, and 1-heptanol between the sodium dodecyl sulfate (SDS) micellar and aqueous phases were measured directly, with the gas chromatographic technique was used to measure the vapor pressure of the alcohols. The standard free energy change of penetration, ΔGp°, of the alcohol from the aqueous to the micellar phase was obtained using the equation: ΔGp°=−RTlnK. There is a linear relation between ΔGp° and the carbon number of the alcohol.

Human mercaptalbumin (HMA) and nonmercaptalbumin (HNA) could be separated by high‐performance liquid chromatography (HPLC) at neutral pH. Using HPLC, the present authors found the nonmercapt‐mercapt conversion (HNA ← HMA) during hemodialysis and the mercapt‐nonmercapt conversion (HMA ← HNA) after hemodialysis in chronic renal failure, indicating HMA as the covalent carrier protein for sulfur‐containing amino acids.

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