Dicoma tomentosa and Alhagi maurorum are the two medicinal plants with fast in-vitro growth. Both the plants have high economic values. Both the plants were investigated on nodal segments and on leaves. The plants were cultured in five different conditions of medium ranging from MS1- MS5. The hormones were used in these mediums in different concentrations. BAP, NAA, Kinetin, and 2,4 D were use. The MS medium in combination with BAP (2.0 and 2.0mg/ml) with NAA 0.1 mg/ml with kinetin 0.25 mg/ml with 2-4 D were taken, where BAP 1 mg/ml with 2 mg/ml of NAA, BAP 2 mg/ml with 0.5 mg/ml of NAA showed better results with callus growth and root-shoot initiation. The best rooting medium found was MS medium supplemented with IAA and IBA 0.5mg/ each. The culture medium was used in different concentrations for estimation of primary metabolites. Maximum protein and lipid percentage were noticed in leaves of both the plants. It can be concluded that both the studied plants have high medicinal importance and can be used as raw material for industry. Keywords: - Dicoma tomentosa; Alhagi maurorum; Plant hormones; MS media.

Dicoma tomentosa and Alhagi maurorum are perennial herbaceous plant having multiple medicinal health benefits. The free radicals are generated in the body as metabolic products of several reactions. These free radicals cause multiple harms to the cell, on their cell wall, to the DNA and cause number of diseases. In the present study, the plants were selected and collected from Rajasthan and were shade-dried. The powder was formed of leaves and stem of both the plants. The methanolic extract was prepared for further studies and the enzymatic and non-enzymatic potential of evaluated through their standard protocols. Dicoma tomentosa and Alhagi maurorum both showed strong antioxidant potential. While comparing both the plants with standard, the stem of Dicoma tomentosa was found having comparatively strong antioxidant potential. Keywords: Alhagi maurorum, Dicoma tomentosa, antioxidant potential, in-vitro study, comparative evaluation.