Biofilm formation helps the microbes to escape host defenses and develop resistance against antimicrobial agents. Early detection of slime production by the Candida species may direct the clinical management. The study was taken with the aim to estimate biofilm formation by Candida species in indwelling catheters and to compare three methods i.e. Congo red agar (CRA) method, Tissue culture plate (TCP) method, and tube method (TM). In this study, we evaluated the reliability of these methods in order to determine most suitable screening method and noted the sensitivity pattern of these isolates with the help of Vitek-2. Our data indicates that the TCP is an accurate and reproducible method for screening and can serve as a reliable quantitative tool for determining bio film formation by clinical isolates of Candida species along with susceptibility testing to reduce resistance pattern.

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