Ionically bound peroxidases (POD) were salt extracted from the pulp of four Indian apple varieties, i.e., Golden delicious HP, Golden delicious JK, Red delicious, and Royal delicious. They were precipitated with chilled ethanol. Thermal treatments of partially purified enzymes were given from 40-70 degrees C for 30 minutes. Golden delicious HP peroxidase showed thermostability at 60 degrees C, while three other peroxidases were observed at 50 degrees C. Phenolic compounds (i.e., caffeic acid, ferulic acids, p-coumaric acid, protocatechuic acid) and metal ions (i.e., Cu2+ and Fe2+) activated all apple peroxidases. However, Mn2+ inhibited the peroxidases from Golden delicious HP, Golden delicious JK, and Red delicious, and a substantial increase was observed in Royal delicious peroxidase. Mg2+ inhibited the peroxidases from Golden delicious HP and Red delicious, but marginal activation was reported in peroxidases from Golden delicious JK and Royal delicious. Zn2+ established stimulation in Golden delicious HP and Golden delicious JK peroxidases, but inhibition was observed in peroxidases in Red delicious and Royal delicious.. Methionine, proline, tryptophan, and valine stimulated all four apple peroxidases, but cysteine showed inhibition in Golden delicious JK.

Although many hydrologic models are available for the estimation of direct runoff from storm rainfall, most models are limited because of their intensive input data and calibration requirements. The Soil Conservation Service-Curve Number (SCS-CN) technique has been applied successfully throughout the entire spectrum of hydrology and water resources, even though originally it was not intended to deal with and solve certain issues such as erosion and sedimentation and environmental engineering. This manuscript includes an updated review of this popular technique with its critical performance analysis under various hydrological applications. The study highlights its provenance and its conceptual and empirical foundations followed by relative significance of the parameter CN and various estimation methods and issues related to the Ia and S relationship. Finally, notable recent advancements available in the literature are discussed for their structural strengths and applicability in real world problems.

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