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Aspergillus niger with mycelium-bound tannase activity was employed to investigate the synthesis of propyl gallate from gallic acid and 1-propanol in organic solvents. The effects of various organic solvents (log P: -1.0-6.6) on the enzymatic reactions showed that benzene (log P: 2.0) was the most suitable solvent. The water content and protonation state of mycelium-bound enzyme both had significant effects on the activity of tannase. The maximum molar conversion (65%) was achieved with 7.3% (v/v) 1-propanol and 5.56 mM gallic acid at stirring speeds of 200 rev/min, 40°C in presence of anhydrous sodium sulfate and PEG-10,000. Enzyme specificity for the alcohol portion (C 1 -C 8 ) of the ester showed that the optimum synthesis was observed with alcohols ranging from C 3 to C 5 .

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