Identification of bioactive compound from alcoholic extract of Acacia farnesiana leguminosae pods by using preliminary phytochemical test or thin layer chromatography and the quantification of total phenolic content by folin-ciocalteu reagent method. shade dried grounded powder of Acacia farnesiana pods was successively extracted with petroleum ether, chloroform and alcohol in soxhlet apparatus, alcoholic compound obtained in more amount as compare to other two extract so alcoholic extract was used for identification of bioactive compound. The alcoholic extract of leguminosae pods indicates the presence of major bioactive compound. Analysis of the alcoholic extract by TLC have identified naringenin from extract, and the total phenolic content in alcoholic extract was found to be 22%(w/w). Therefore the present study deals with qualitative analysis of alcoholic extract of legumae pericarp (pod wall) of Acacia farnesiana L. In which we analyze various phytochemical which are useful for contoling various diseases TLC method used for identification of the content of naringenin from active extract of Acacia farnesiana pods. It is concluded that, legume pods contain maximum phytoconstituents or phenolic content.

Betalains have potential as the natural colourant and functional food with pharmaceutical purposes in food applications. The total betalain content of Basella rubra L. grown at Kalaburagi region is evaluated for the first time. The present study is carried out to determine the pigment stability and antimicrobial activity of fruits of Basella rubra. Total betalain content of fruits was evaluated and it was found to contain a high amount of 48.15 mg betacyanins and sparing amount of 2.53 mg betaxanthins and total betalains account for 50.69mg/100g of fresh fruit pulp. The influence of different additives on the colour stability of pigment was evaluated. More than 80% pigment colour is stable in the presence of 5% sucrose at 4 °C under dark condition. The betalain extract of Basella rubra L fruits showed significant antimicrobial activity against S.typhi, Aspergillus flavus, Microphomia fungi and Rhizopus stolonifer. It is for the first time that the total betalain content and anti-microbial activity of Basella rubra cultivar grown in Hyderabad Karnataka region of Karnataka, India is reported.