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Aim: RhoA/Rho kinase pathway is essential for regulating cytoskeletal structure.Although its effect on normal neurite outgrowth has been demonstrated, the role of this pathway in seizure-induced neurite injury has not been revealed. The research examined the phosphorylation level of RhoA/Rho kinase signaling pathway and to clarify the effect of fasudil on RhoA/Rho kinase signaling pathway and neurite outgrowth in kainic acid (KA)-treated Neuro-2A cells and hippocampal neurons.Method: Western blotting analysis was used to investigate the expression of key proteins of RhoA/Rho kinase signaling pathway and the depolymerization of actin. After incubated without serum to induce neurite outgrowth, Neuro-2A cells were fixed, and immunofluorescent assay of rhodamine-phalloidin was applied to detect the cellular morphology and neurite length. The influence of KA on neurons was detected in primary hippocampal neurons. Whole-cell patch clamp was conducted in cultured neurons or hippocampal slices to record action potentials.Result: KA at the dose of 100-200 μmol/L induced the increase in phosphorylation of Rho-associated coiled-coil-containing protein kinase and decrease in phosphorylation of Lin11, Isl-1 and Mec-3 kinase and cofilin. The effect of 200 μmol/L KA was peaked at 1-2 hours, and then gradually returned to baseline after 8 hours. Pretreatment with Rho kinase inhibitor fasudil reversed KA-induced activation of RhoA/Rho kinase pathway and increase in phosphorylation of slingshot and 14-3-3, which consequently reduced the ratio of G/F-actin. KA treatment induced inhibition of neurite outgrowth and decrease in spines both in Neuro-2a cells and in cultured hippocampal neurons, and pretreatment with fasudil alleviated KA-induced neurite outgrowth inhibition and spine loss. Conclusion:These data indicate that inhibiting RhoA/Rho kinase pathway might be a potential treatment for seizure-induced injury.

In the post-epidemic era, luxury goods have seen a decrease in production and sales abroad, while sales in China have tended to rise and even increase in price. The paper has examined the current situation and problems from the perspectives of sales channels, localization, brand building investment and segmentation of target customers, and made suggestions on marketing strategies in terms of enhancing the public welfare image of brands, taking social responsibility, promoting digital marketing transformation, standardizing channel management, increasing investment in second and third tier cities and focusing on young target customers. The company put forward marketing strategy suggestions. In particular, for the digital marketing transformation, suggestions were made to strengthen the construction and promotion of the official website, promote the diversified sales model of the e-commerce platform and give full play to the influence of social media. At the same time, it was suggested that major luxury brands could strengthen cross-border brand alliances and production line cooperation to enhance brand awareness and reputation, increase the production capacity of each partner, and develop greater market space.