In patients with CCHF, especially children, hematuria, proteinuria, oliguria and azotemia could have been found by urinalysis. Moreover, because of the fact that proteinuria and urine neutrophil gelatinase-associated lipocalin (uNGAL) levels could increase in children with CCHF; thus, monitoring of renal involvement related to CCHF by measurement of urine total protein and uNGAL is recommended. Please cite this paper as:Khazaei Z, Darvishi I, Amiri M, Sohrabivafa M, Kamran S. Crimean Congo hemorrhagic fever: a brief report regarding kidney involvement. J Renal Inj Prev. 2018;7(3):129-131.
Purpose Colorectal cancer (CRC) is one of the most prevalence malignancies in a different society with a high rate of death. The KRAS and p38α axes have critical roles in the development, migration, and growth of numerous tumors, such as colorectal malignancy. KRAS mutation acts as an oncogene in various cancers and is correlated with the poor prognosis in colorectal tumors. Also, p38α plays different roles and exhibits tissue-dependent activity. In some tissues act as an oncogene while in others act as a tumor suppressor. In this research, we try to understand the effect of the P38α and KRAS genes suppression by specific siRNAs on the SW480 cell line progression. Methods We evaluate the impact of the P38α and KRAS gene knockdown by special siRNA on the growth and development of the SW480 cell line. SW480 cell line was treated with KRAS and P38α siRNAs, and the cell viability, gene expression, migration ability, and rate of apoptosis were evaluated with MTT assay, real-time PCR, scratch test, and flow cytometry. Results After treatment of the cancer cell with KRAs and P38α siRNAs, cell viability reduced to 29.16%. Also, the expression levels of the KRAS and P38α genes reduced to 26.34% and 16.06%, respectively. Apoptosis rate after combination therapy with KRAS and P38α siRNAs increased to 72.1. Also, we found that these siRNAs suppress cell migration in SW480 cell lines. ConclusionThe current study showed that combination therapy with p38α and KRAS siRNA may be considered a novel therapy for colorectal tumor in future. KeywordsColorectal cancer • KRAS • P38 • siRNA Abbreviations siRNA Small interfering RNA KRAS Ki-ras2 Kirsten rat sarcoma virus MTT 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide EGFR Epidermal growth factor receptor MAPK Mitogen activation protein kinase NF-κB Nuclear factor kappa light chain enhancer of activated B cells PP2AC Protein phosphatase 2 catalytic subunit alpha gene
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.