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The present investigation entitled Effect of different potting media, bio-agents and organic amendments on the nutrient uptake, Percentage of saleable seedlings and root rot disease of acid lime seedling cv. Balaji was carried out during 2016-2017 at AICRP on Fruits, Citrus Research Station, Tirupati. The experiment was laid out completely randomized design with 21 treatments. The result of the present investigation indicated that, the medium combination soil, sand, cocopeat @ 1:1:1 v/v, neem cake (20g) with AM F@ 5g had given significantly highest N, P, K content in leaves and stem at 150 days old seedlings. Lowest leaf and stem N, P, K content was observed in treatment combination of FYM +Sand +Soil and the number of such saleable seedlings was recorded at 150 days after transplanting. In two months old seedlings maximum saleable plants were observed in treatment-18 (soil, sand, cocopeat @ 1:1:1 v/v, neem cake (20g) with AMF@ 5g). In case of three months old seedlings maximum percentage of saleable seedlings was observed in T 12 containing (soil + sand + vermicompost + Arbuscular mycorrhiza (5g) + neem cake 20g), similarly in four months old seedlings maximum percentage of saleable seedlings was observed in T 12 whereas minimum was recorded in T 1 and there was no incidence of root rot disease in citrus seedlings among different intervals of data recorded. K e y w o r d sNPK, Acid lime, Arbuscular mycorrhiza fungi, Azotobacter