Yograj guggulu vati (YGV), a polyherbal formulation is recommended for the management of diseases like arthritic, anodyne or analgesic, spasm, muscle relaxant, flatulence, digestive problem, cough, hyperglycaemia, fat burner and obesity. Though Yograj guggulu vati is widely used for the treatment of diseases in Ayurvedic System of Indian Medicine, but till date, it’s Phenolic and flavonoids contents and contamination studies have not been carried. In the present article, we evaluated the total phenolic and flavonoids contents and contamination of YGV. Total phenolic contents were evaluated by Folin Ciocalteu reagent. Aluminum chloride colorimetric method was used for the determination of total flavonoid contents. Contamination study such as microbial load was also performed. Microbial load study revealed that total bacterial counts and total fungal counts were under limits. The total phenolic content and total flavonoid content were 190.16 mg/g and 20.87 mg/ g dry extract respectively. Microbial load studies showed that the formulation has a good quality and purity. Presence of abundance phenolic and flavonoids compound indicated that YGV can be used for different biological activities. Keywords: Microbial load, Yograj Guggulu Vati, total phenolic contents