Background: Pulmonary function assessment has achieved a lot of importance nowadays owing to a steep rise in air pollution. Lung function parameters tend to have a relationship with lifestyle such as regular exercise and non-exercise. Hence the present study was under taken to assess the effects of exercise in athletes on respiratory system and compared with sedentary group. Aims & Objective: To compare the differences in pulmonary function test among the athletes and sedentary group. Materials and Methods: A total of 152 subjects comprising athletes and sedentary were assessed for pulmonary function test. The parameters used as determinants of lung function were FVC, FEV1, FEV3, PEFR and FVC/FEV1 ratio were recorded as per standard procedure using Medspiror. Results: Pulmonary Function Profile was analyzed and compared between the study groups. In our study the athletic group were having higher mean of percentage value of FVC 88.0 ± 12.8%, FEV1 of 86.8 ± 22.0%, FEV3 of 86.5 ± 13.7 %, PEFR of 93.0 ± 12.8% and FEV1/ FVC ratio of 92.1 ± 4.4% as compared to sedentary group. Conclusion: The FVC, FEV1, FEV3, PEFR and FEV1/FVC ratio were higher in athletes than in the normal sedentary control individuals. This study suggests that regular exercise has an important role in determining and improving lung functions.