The pharmacokinetics of FA-Pr-Al in combination were closely related its pharmacodynamics in treating ischemia/reperfusion injury, and the components of FA-Pr-Al may have a synergistic pharmacological effect. Astragaloside may play a more pronounced role in regulating IL-1βand NPY levels compared with puerarin or FA.

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Objective:Immersion induced hydrostatic pressure on thoracic cavity and limbs is one kind of cardiac overload model in simulating microgravity. We evaluated immersion hemodynamic parameters, investigated in vitro stretching induced aortic intact wall windkessel adaptations in whole-body immersion mice. Method: Kunming mice were divided into immersion group (n=20) and control group (n=10) randomize. In immersion group, mice further divided into immersion group (n=10) and immersion with weight-bearing group (5% of body weight, n=10). The immersion kept 20 min/day in warm water and continues for 1 week. The hemodynamic parameters were collected by left carotid artery cannulation and analyzed by biological signal acquisition system. Calculi of percussion wave were calculated with integral mode to determine left ventricle stroke volume. In vitro aortic wall were prepared to a vascular ring and mechanical stretching the preparation to 1 g as the initial load. The preparations were further stretched by a micro-adjusting tuner with the interval of 5 min which gradually increasing 1 mm in each step. The stretching induced passive tension, where after, stress relaxation duration and myogenic spontaneous contraction were analyzed. In a separated preparation, myogenic contraction changes investigated in 0.05% Nitrendipine treated preparation. Result: Mice carotid arterial pressure waveforms have significantly changed, however, mean arterial pressure were slightly increased but not significantly (immersion 9.14 ± 0.63 kPa, control 8.40 ± 0.97 kPa). The calculated stroke volume increased significantly (immersion 13.96 ± 0.12 µl/min, control 5.14 ± 0.69 μl/min, respectively). In vitro mechanical stretching induced less myogenic spontaneous contraction, stress relaxation period were shortening. Pre-treatment of 0.05% Nitrendipine induced more myogenic spontaneous contraction that revealed the spontaneous contraction was not solution Ca 2+ influenced. Conclusion: Immersion stress improved left ventricle functions that reflected in rapid ejection and isodiastolic period, suggested aortic windkessel properties were improved in microgravity simulation induced cardiac overload.

In order to improve the extraction technology of Polysaccharides from Ophiocordyceps sobolifera and its antioxidant activity, and an optimal enzyme method combined response surface methodology was provided in this paper. Take the Polysaccharides extraction rate processed by protease, cellulase and pectase as response value, while the amount of enzyme, enzymolysis temperature, enzymolysis time, and its pH as response factors, the experimental design of four factors and three levels was applied to Box-Behnken central composite method. The antioxidant activity of the extracts was determined by spectrophotometry. The optimal conditions for the extraction were found to be: the amount of enzyme of 1.5%, enzymolysis temperature at 49 , enzymolysis time of 150 min, pH at 4.8 and ratio of solid to solvent of 1:20 for Ophiocordyceps sobolifera Polysaccharides. Regression analysis showed that 1.2% relative error between experimental values and the model employed. The elimination of hydroxide free radical was at 44.51%. These indicated that optimization of extracting technology condition be reliable, which based on response surface method, and the extraction has a high antioxidant activity.